Intelligent Rotational Behavior Monitor for Experiment Animal / 中国比较医学杂志

Objective to develop a rotation monitoring device to monitor animal rotation during experiments.Methods Use serial port controls,USB and rotary encoder et al.modern computer cience,with the combination of traditionally experimental methods and the software designed in VC language.Results A mini animal rotation monitor was developed,which could continuously monitor animal rotation and intelligently display the rotaion.conclusion Statistic analysis of experimental data proofs the rationality and effectiveness of the apparatus.The rotational behavior monitor can play an importance role in research into the pathogenesis of Parkinson's Disease(PD)and screening the anti-PD drugs.

Chronological Changes in the Rotational Behavior in Response to Apomorphine Administration in 6-Hydroxydopamine Parkinsonian Rat

BACKGROUND: Apomorphine-induced rotational behavior of unilateral 6-hydroxydopamine (OHDA) lesioned rat is widely used to develop anti-Parkinsonian treatments including drugs, neuroprotective therapy, and neural graft. Time course of changes in rotational behavior after lesioning, however, has not been fully elucidated. The aim of this study was to observe the chronological changes in the rotational response and to find the optimal period when this model is used for investigation of various therapies. METHODS: 6-OHDA was stereotaxically delivered to the unilateral substantia nigra in 13 rats. Rotational responses to apomorphine administrations were counted in the rotomotor on 2, 4, 8, 12, and 14 weeks after lesioning. RESULTS: The total turns for two hours increased continuously up to eight weeks, and then plateaued. CONCLUSIONS: Apomorphine-induced rotations increase until eight weeks after 6-OHDA lesioning. Therefore, this Parkinsonian model should be used at least eight weeks after lesioning. Even though priming was not excluded as an explanation in the experiment, we reason that progressive degeneration of dopaminergic neurons may explain the chronological changes in rotational behavior.

A Study on the Behavioral Properties of the Rat Parkinsonian Model

Recently the reports of the autologous grafting of adrenal medullary tissue into the brain of parkinsonian patient have given the wide attention to the neurosurgeons as well as other clinicians, because the current therapeutic modalities are either imperfect or palliative. Although neural grafting of adrenal medullary tissue of fetal brain which can supply the dopamine seems to be a ideal form of treatment theoretically, many problems must be overcome for this approach to be a routine procedure. Authors made the rat parkinsonian model by destroying the substantia nigra and nigrostriatal fiber selectively with 6-OHDA. And abnormal behaviors and growth patterns were observed and studied using rotometry, T-maze and metabolic cage. With the results, some parameters which would be useful in further experiments could be established.

The antagonistic effect of notoginsenoside-Rg_1 injected in striatum on immunoinflammatory injury in rat model of Parkinson's disease / 中国免疫学杂志

Objective:To study the effect of notoginsenoside-Rg 1 on immunoinflammatory injury in rat model of Parkinson′s disease.Methods:The rat model of Parkinson′s disease was established and treated with notoginsenoside-Rg 1 by injecting in the striatum.The change of rotational behavior,levels cytokines in striatum and splenocyte proliferation were measured.Results:After treatment with notoginsenoside-Rg 1 the rotational behavior and levels of TNF-?,IL-1?,IL-6 were decreased.The splenocyte proliferation was increased.Conclusion:Notoginsenoside-Rg 1 can regulate the immunological function and reduce the pathological injury to dopaminergic neuron caused by immunoinflammatory reaction.